In-Vitro Antimicrobial Synergistic and Anti-Tb Debnath Subal1, Kumar G. Ganesh1, Chandu Atul N.2 1Srikrupa Institute of Pharmaceutical Sciences, Vil. Velkatta, Kondapak (MDL), Dist. Medak, Siddipet, Andhra Pradesh – 502 277 2University Institute of Pharmacy, Pt. Ravishankar Sukla University, Raipur, Chhattisgarh, India *Corresponding Author: Santhosh Kumar C. Srikrupa Institute of Pharmaceutical Sciences, Vil. Velkatta, Kondapak (mdl), Dist. Medak, Siddipet, Andhra Pradesh – 502 277. Email: santhoshkcrpharma29@rediffmai.com Phone: 08008928923
Online published on 21 March, 2013. Abstract The objective of the present study was to evaluate the synergistic antimicrobial and anti-tubercular activities of Opuntia dillenii aqueous methanolic extract. Antibacterial study was carried out by plate hole diffusion or agar well diffusion assay to determine the growth inhibition of bacteria. Antifungal activity was performed by the use of Saubouraud dextrose agar medium (SDA). The synergistic activity study was calculated by means of Cup plate method (Kirbauy bauer technique) using two wells in a plate. The aqueous methanolic plant extract of Opuntia dillenii (500 μg/ml) was used in combination with oxytetracycline (500 μg/ml). Anti-tubercular assay was performed using Micro plate Alamar Blue Assay (MABA) using the suspension of Mycobacterium tuberculosis H37Rv strain. The concentrations of plant extract were used are 1000 μg/ml, 500 μg/ml, 250 μg/ml, 125 μg/ml, 62.5 μg/ml. The medicinal plant appear to have a broad antimicrobial activity spectrum, they could be useful in antiseptic and disinfectant formulation as well as in anti-tubercular activity. Among the various micro organisms, the aqueous methanolic extract was more active against Micrococcus leuteum, Proteus mirabilis. In antifungal activity of the aqueous methanolic extract shows positive results for all fungus. The antitubercular activities were compared with standard drug Rifampicin. The aqueous methanolic extract was having more percentage inhibition when compared to other extracts. Top Keywords Opuntia dillenii, Mycobacterium tuberculosis, Micro Plate Alamar Blue Assay. Top |