Screening of Immunomodulatory Activity of Extracts of Seeds of Jatropha curcas Linn. On Human Neutrophils Hugar DS1, Heroor Sanjeev1,*, Hussain Zakir2 1HKES's College of Pharmacy, Gulbarga – 585105 2Luqman College of Pharmacy, Gulbarga – 585102 *Corresponding Author: Mr. Sanjeev Heroor, Lecturer Department of Pharmacognosy and Phytochemistry HKES's college of pharmacy Gulbarga – 585105, ssheroor@gmail.com, Mob. No. +919972080880 Fax No.08472-244662
Online published on 21 March, 2013. Abstract Jatropha curcas Linn. (Euphorbiaceae) traditionally claimed to be used as tonic, tissue builder and stimulant, the present work was undertaken to prove scientifically its tonic property as immunomodulator (immunostimulant) on human neutrophils by some in-vitro models, viz.-Nitroblue tetrazolium (NBT) dye test, phagocytosis of candida albicans and chemotaxis assay at different concentrations – 10μg/ml, 20μg/ml, 40μg/ml, 100μg/ml and 1000μg/ml (undiluted extract) of aqueous and petroleum ether(40–600 c) extracts of seeds of the plant. The extracts at concentrations of 1000μg/ml (undiluted extract) and 100μg/ml showed 7080% of phagocytic and chemotactic activities when compared to normal and positive controls in the parameters studied. However, the activity was lower at concentrations 40μg/ml, 20μg/ml and 10μg/ml. Preliminary phytochemical investigation showed the presence of flavonoids, tannins, carbohydrates, saponins and amino acids in the extracts; hence these compounds might be responsible for immunomodulatory (immunostimulant) activity. Top Keywords Jatropha curcas, Immunomodulatory (immunostimulant), NBT, candida albicans, chemotaxis. Top |