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Research Journal of Pharmacognosy and Phytochemistry
Year : 2023, Volume : 15, Issue : 4
First page : ( 281) Last page : ( 287)
Print ISSN : 0975-2331. Online ISSN : 0975-4385.
Article DOI : 10.52711/0975-4385.2023.00044

Synthesized green silver nano particles of Herpetospermum darjeelingense shows enhanced In vitro antimicrobial, antioxidant and hepatoprotective activity

Chakraborty Sourav1,2, Saha Sumedha1, Bhattacharya Malay1,*

1Molecular Biology and Tissue Culture Laboratory Department of Tea Science, University of North Bengal, Siliguri, Darjeeling, West Bengal, India, 734013

2Post Graduate Department of Botany, Darjeeling Government College, Darjeeling, West Bengal, India, 734101

*Corresponding Author E-mail: malaytsnbu@gmail.com

Online Published on 30 January, 2024.

Abstract

Silver nanoparticles (AgNP’s) are 1 - 100nm sized constituents providing additional surface area in formation of plentiful coordinate bonds with various ligands. Green mode of AgNP’s synthesis has aided in development of advanced formulations towards treating complex human ailments. Herpetospermum darjeelingense (C.B.Clarke) H. Schaef. and S.S. Renner is an under investigated endemic ethnomedicinally significant cucurbit climber with no work based on synthesis and exploration of biogenic AgNP’s being conducted previously employing its solvent extracts. This paper aims to provide insights on the green synthesized AgNP’s besides studying its antimicrobial, antioxidant and hepatoprotective efficacy. Methanolic extracts of H. darjeelingense were utilized to synthesize AgNP’s via reduction of silver nitrate following UV-Visible spectroscopic and scanning electron microscopic characterization depicting surface plasmon resonance bands at around 430nm wavelength with spherical to cubical shaped AgNP’s ranging between 40-80nm. The methanol based sample extracts did not portray any inhibition zone in antimicrobial assay with the AgNP’s showing considerable zone of inhibition against Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus indicating anti-microbial effectiveness of silver nano formulations. The highest free radical scavenging potential was shown by 50mg/ml (91.99%) silver nano formulation with minimal being recorded in 12.5mg/ml (50.11%) nanoparticle concentrate including a noted gradual increase in anti-lipid peroxidation ability from 12.5mg/ml (7.157±0.08273mg/ml Tocopherol equivalent) to 50mg/ml (18.128±0.03152mg/ml Tocopherol equivalent) nanoparticle formulation clearly portraying statistically significant elevated results in comparison to plain sample extracts depicting its anticipated usage in pharmaceutical applications concerning antibacterial, antioxidative and hepatoprotective features.

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Keywords

Herpetospermum, Silver nanoparticles, Antimicrobial, Antioxidant, Hepatoprotective.

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