Effect of Lecithin on CFA Induced Rheumatoid Arthritis in Female Wistar Rats Ali Shaik. Liakhat*, Reddy D. Subba, Sravani K., Sowmya S. Sri, Joshi Rahul Nirmala College of Pharmacy, Atmakur, Guntur, Andhra Pradesh, India *Liakhat761@gmail.com
Online published on 27 September, 2019. Abstract Rheumatoid arthritis (RA) is a chronic systemic autoimmune inflammatory disease that affects mainly the small joints of the hands and feet. RA is one of the most common inflammatory joint diseases and causes premature mortality, disability and compromised quality of life. The overall worldwide prevalence is 0.8% and steadily increases to 5% in women over the age of 70. RA is two to three times more common in women compared to men. In India the prevalence has been estimated to be 0.7%. Accordingly the main objective of the present study was to evaluate the activity of lecithin using in-vivo model, on CFA induced rheumatoid arthritis in Wistar rats of weights 250–260 g were selected & divided into 6 groups each of 6 randomly paw edema. The test and standard doses were administered orally. The paw edema was measured by using vernier calipers. At 21st day the animal blood collected through retro orbital route and haematological parameters were estimated, After the induction of CFA, the animal develops inflammatory responses and shows animals sacrificed by giving high dose of anesthesia then left hind paw was dissected and performed histopathology studies. The observations such as paw volumes recorded on 1st, 4th, 8th, 14th, and 21st days-after adjuvant injection. The increase in the paw volume (arthritis one of the signs enhanced) observed in CFA induced Arthritis control group which indicates primary and secondary arthritic lesion. The evaluation finished on the 24th day showed that the standard and lecithin treatment had significantly reduced the paw volumes and increase in body weights in the respective treatment groups as compared with the CFA control group. The percentage inhibition of paw volumes on 24th day, standard Indomethacine (10mg/Kg, p.o.) was 40%, 100mg/kg, 300mg/Kg and 600mg/Kg doses of lecithin were 34%, 36% and 40% respectively compared against control. The haematological parameters such as an increase in the WBC count, decrease in RBC count and increased Hb % were in CFA induced group but in treatment groups, in lecithin-and standard treated groups these altered parameters were restored almost to normal levels, so these results indicates that lecithin may have anti-arthritic activity. Top Keywords CFA (Complete Freund's adjuvant), Rheumatoid Arthritis, Indomethacin, lecithin. Top |