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The Journal of Indian Botanical Society
Year : 2010, Volume : 89, Issue : 3and4
First page : ( 375) Last page : ( 378)
Print ISSN : 0019-4468. Online ISSN : 2455-7218.

In Vitro screening of Callus Cultures of Maize Treated with Toxins of Helminthosporium Species

Sukhwal R., Trivedi R., Purohit S.D.*

Post Box No. 100, Plant Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, 313 001, India

*Corresponding author; Fax: + 91-294-2410300, E-mail: sdp_56@hotmail.com

Online published on 25 April, 2016.

Abstract

Callus cultures obtained from three varieties of maize viz. ‘Malan ’, ‘Ganga-5 ’and ‘VL-42 ’were screened for their response towards toxin extracts of Helminthosporium maydis and H. turcicum. The variety ‘Malan ’showed susceptibility towards both the pathogens whereas variety ‘Ganga-5 ’showed fairly good resistance/tolerance to H. maydis as compared to H. turcicum. However, variety ‘VL-42 ’showed resistance to the toxic extracts of H. Turcicum while it was found susceptible to H. maydis. Callus cultures provided good system and could be applied for large-scale screening of germplasm.

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Keywords

Callus, germplasm, in vitro screening, pathotoxins.

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