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The Journal of Indian Botanical Society
Year : 2010, Volume : 89, Issue : 3and4
First page : ( 281) Last page : ( 287)
Print ISSN : 0019-4468. Online ISSN : 2455-7218.

Protease, Peroxidase and Lipoxygenase Activities During Salinity Stress on Early Seedling Growth of Groundnut (Arachis hypogaea L.)

Basha S.K.M., Fareeda G., Savithramma N.*

Dept.of Botany and Dept.of Biochemistry, S.V. University, Tirupati-517502, Andhra Pradesh, India

*E-mail: Prof.Savithri@yahoo.in

Online published on 25 April, 2016.

Abstract

The present research work was carriedout to study the protease, peroxidase (POX) and lipoxygenase (LOX) activities to elucidate the adverse effect of NaCl salinity and its amelioration by CaCl2 during early seedlings growth of two groundnut cultivars (cv. TPT-2 and cv. TCGS-29). The cv. TCGS-29 showed higher level POX activity than cv. TPT-2. Addition of CaCl2 to NaCl stressed seedlings decreased the level of protease activity than the NaCl treated seedlings.

NaCl treatment caused decrease in the level of LOX activity when compared to the seedlings treated with either CaCl2 and its combination with NaCl. LOX showed a single peak with an UV absorption maximum at 235 nm on HPLC. cv. TPT-2 is stress tolerant than cv. TCGS-29. 30 mM and 15 mM Ca2+ treatment alleviated the stress effect in cv. TPT-2 and TCGS-29 respectively.

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Keywords

Lipoxygenase, Peroxidase, Protease, Salinity stress.

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