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Journal of Forensic Medicine and Toxicology
Year : 2023, Volume : 40, Issue : 2
First page : ( 11) Last page : ( 14)
Print ISSN : 0971-1929. Online ISSN : 0974-4568.
Article DOI : 10.5958/0974-4568.2023.00032.7

Cytomorphic analysis of labial mucosal cells as a predictor of age and sex in forensic odontology

Baghel Shilpi1, Sreedhar Gadiputi2, Gupta Shalini1,*, Devi Priya1

1Department of Oral Pathology & Microbiology, King George's Medical University, Lucknow, India

2Department of Oral Pathology and Microbiology, KIMS Dental College and Hospital, Amalapuram, India

*Corresponding Author Dr. Shalini Gupta, Professor, Department of Oral Pathology & Microbiology, King George's Medical University, Lucknow, Uttar Pradesh, India - 226003, Email: sgmds2002@yahoo.co.in

Online published on 1 April, 2024.

Abstract

Background

Establishing individuality is an imperative aspect of any investigative procedure. Sex and age determination in Forensic Odontology is the application of dental principles for legal issues in crimes like theft, sexual assault, murder etc.

Aim

Correlate the Cytoplasmic area, nuclear area, N/C ratio for estimation of age and sex of an individual as an adjunction forensic(crime) investigation in the Lucknow population.

Methodology

In this study, 100 labial smears were collected from normal individuals, dividing them into four age groups. (Group1:20-30,Group2: 3040, Group3: 40-50,Group4: 50-60). The collected smear samples were stained using Papanicolaou stain. The average sizes of the cells and nucleus were measured using image proplus image analysis software.

Result

N/C ratio, NA and CA were more in females when compared to males. All the above parameters reduced with progressing age in both the sexes. All parameters showed statistically significant difference in different age groups.

Conclusion

Cytomorphometric evaluation of exfoliated oral cells proves reliable tool for correlation of age.

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Keywords

Papanicolaoustain, Nuclear/cytoplasmic ratio, Nuclear area, Mean cell area, Cytomorphometry.

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