Raid screening of antityrosinase compounds from fungi Krishnaveni R1,2, Rathod Vandana2,*, Kulkarni Prema2, Rao Shubaand Hema 1Dept of Biotechnology, University of Agricultural Sciences, Dharwad 2Department of Microbiology, Gulbarga University, Kalaburagi *E-mail: drvandanarathod17@rediffmail.com
Online published on 30 November, 2019. Abstract Tyrosinase inhibitors are involved in controlling the undesired browning in fruits, vegetables and marine foods and also they used as whitening agents. The research in finding tyrosinase inhibitors drawn attention as there is a need in finding new inhibitors because the present tyrosinase inhibitors are having side effects such as carcinogenic properties. The screening of different fungies done through plate assay technique using dual assay technique on enriched PDA and inibitory studies done by using spectrophotometric assay, where partially purifired tyrosinase from Acremonium rutilum was used, revealed that A.niger-1 and A.ornatus showed 98.3 and 98.1 per cent inhibition, which were highest as compared to other screened fungi. Plate assay method appears to be the best and rapid method for tyrosinase inhibitors, as all isolates screened by plate assay has shown inhibition to partially purified enzyme which were used for plate assay. A.ornatus showed a strong positive reaction with Fecl3 test indicating that the compound is kojic acid which was separated by TLC with a standard kojic acid and the separated compound again showed 98.5 per cent tyrosinase inhibition. Kojic acid was identified as a potent fungal tyrosinase inhibitor from A.ornatus. A.niger -1 didn't show any of the compounds tested and require a detail study. When the percent inhibition of synthetic kojic acid was compared with naturally screened and identified kojic acid, the percent inhibition was 91 per cent with synthetic IC 50 1mM, where as it was 98.5 per cent inhibition which is appreciable with natural kojic acid irrespective of the concentration. Top Keywords Aspergillus ornatus, kojicacid, tyrosinase inhibitory activity. Top |