Bud chip method: A potential In vitro technology for bajranapier hybrid (Pennisetum glaucum L. X P. purpureum Schum.) for sett multiplication Senthilkumar D.1,*, Paramasivam A.1, Ramachandran M.1 1Livestock Farm Complex, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu-614 625, Tamil Nadu, India *Corresponding Author: D. Senthilkumar, Livestock Farm Complex, Veterinary College and Research Institute, Orathanadu-614 625, Tamil Nadu, India, Email: senthilkumar.d@tanuvas.ac.in
Online published on 5 July, 2024. Abstract Background Forages of high quality are the most valuable commodity of any livestock activity and they serve as the basis for most of the rations in a forage-based diet. The lack of good quality seedlings/seeds, notably improved varieties, is the primary reason for the slow adoption of improved forage production technologies. Traditional establishment of bajra napier forage incurs more labour costs, harvesting sett material, transporting, preparing two budded setts and carrying to the planting area. Nearly three to four tons of green leaves stem materials are wasted for planting one hectare of area. In this situation, an alternate method is required to produce high-quality seedlings in a short period. This research objective compared single node bud chip seedling in different protray cell cavities and pot mixtures. Methods The green shade net field study was carried out between 2019 and 2020, the effect of different pro-tray sizes (50, 60 and 98 cell cavities) and potting mixture (red earth, cocopeat, farmyard manure, vermicompost and root growth promoters like phosphobacteria bio-fertilizer and indole acetic acid (IAA) was taken in different combination for the establishment of Bajra Napier hybrid variety Co (BN)-5 seedling by using single node bud chip technology. The observation was taken up to 30 days in related to seedling growth and development. The observed parameter was statistically analysed in SPSS software. Result Among the three different protray cavities and five different pot mixtures, 60 cavity protray with red earth + cocopeat + vermicompost (2:1:1) results revealed significant differences (p>0.05) in seedling growth parameters among the different protray and growing media. The maximum seedling growth parameters like germination, numbers of the leaf, plant height, leaf length, width and root length (94.4 per cent, 8.17, 39.12 cm, 38.14 cm, 2.5 cm and 30.8 cm) were observed at 30 days aged seedlings followed by red earth + cocopeat + farmyard manure (2:1:1 ratio) and treated with IAA substance in 60 cavity protray. Top Keywords IAA, Pot mixture, Protray, Single node bud chip bajra napier, Vermicompost. Top |