Evaluation of antioxidant potential of taurine in tris egg yolk citrate extender in surti buck semen preserved at refrigerated temperature Kumar David1,*, Khasatiya Chandubhai T.1, Chaudhary Sandhya S.2, Singh Virendra Kumar2 1Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Kamdhenu University, Gandhinagar-382 010, Gujarat, India 2Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Navsari Kamdhenu University, Gandhinagar-382 010, Gujarat, India *Corresponding Author: David Kumar, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Kamdhenu University, Gandhinagar-382 010, Gujarat, India, Email: davidkumarnau4@gmail.com
Online published on 4 July, 2024. Abstract Background Taurine supplementation in tris egg yolk citrate (TEYC) extender can improve antioxidant defense and reduce motility degeneration rate in semen of Surti buck preserved at refrigeration temperature. Present study has evaluated antioxidant effect of different concentrations of taurine in TEYC extender on oxidative stress, antioxidant defense and motility degeneration rate in Surti buck semen preserved at refrigerated temperature. Methods Four Surti bucks (age >2 years) were selected. Total 64 ejaculates (16 per buck) were collected during a period of 8 week. Post-collection they were pooled and stored at refrigeration temperature after dividing into 5 groups based on different taurine concentrations in TEYC extender viz. 0 mM (control T1), 25 mM (T2), 50 mM (T3), 75 mM (T4) and 100 mM (T5) taurine maintaining final concentration of 200×106 sperm/ml (pH 6.5-6.8). Evaluation of motility degeneration rate at 24, 36 and 48 hours and glutathione as well as lipid peroxidation (MDA) at 0 and 48 hours was done. Result Supplementation of taurine @50 mM in TEYC extender caused post-chilling significant increase in reduced glutathione (GSH) levels, lowering of lipid peroxidation (in terms of MDA production) and reduction of motility degeneration rate (MDR)% in semen of Surti bucks. It improved antioxidant defense thereby maintaining good quality of semen. Top Keywords Antioxidant, Refrigerated, Surti buck semen, Taurine, TEYC. Top |