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Asian Journal of Dairying & Foods Research
Year : 2013, Volume : 32, Issue : 1
First page : ( 83) Last page : ( 88)
Print ISSN : 0971-4456. Online ISSN : 0976-0563.

Efficacy of Caseinophosphopeptides in the remineralization of demineralized teeth (In-vitro studies)

Nagamani A., Hiremath Jayashri P., Narasimhamurthy

Department of Dairy Chemistry, Dairy Science College, Karnataka Veterinary, Animal & Fisheries Sciences University, Bangalore- 560 024, India

*Corresponding Author's E-mail: nagudscb@gmail.com

Online published on 16 September, 2013.

Abstract

The remineralization efficacy of the Caseinophosphopeptides (CPPs) obtained from skim milk casein hydrolysates using neutrase and trypsin enzymes on the demineralized tooth were explored. Casein hydrolysates produced by neutrase and trypsin yielded hydrophobic and relatively hydrophilic CPPs respectively. Toothpastes (T2, T3, T4, T5) were prepared by incorporation of 3 and 4% CPPACP complexes obtained from hydrophobic and hydrophilic CPPs while Placebo (T1) without CPPACP complex. Demineralized teeth were treated with toothpastes for a period of 8 weeks. The photographic images of the teeth before and after treatment showed visible differences in the demineralized region after treatment with T2, T3, T4, T5 indicating their remineralization efficacy. Toothpastes containing CPPs (T3 and T5) at 4% showed higher remineralization ability than the toothpastes containing CPPs (T2 and T4) at 3% and placebo toothpaste. Remineralization efficacy of the toothpastes revealed the bound calcium percentage on teeth as 0.42, 0.56, 0.48, 0.65 and 0.01% in case of T2, T3, T4, T5 and T1 respectively which confirmed maximum remineralization efficacy by toothpastes containing 4% CPP-ACP obtained from trypsin.

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Keywords

Anticariogenic property, Caseinophosphopeptide-Amorphous calcium phosphate complex, Demineralization, Remineralization, Toothpaste.

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