Isolation of Bacteria, their Sensitivity and Resistance Pattern in Bovine Mastitis Saravanajayam M.1,*, Ravikumar R.2, Balasubramaniam A.1, Palanivel K.M.3 Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai-600051, Tamil Nadu 1Veterinary University Training and Research Centre, Perambalur 2Avian Disease Laboratory, Thalaivasal, Salem District, Tamil Nadu 3Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal *Corresponding author. E-mail: saravet78@gmail.com
Online published on 2 April, 2016. Abstract The study was designed to investigate prevalence of microbes associated with clinical mastitis and their pattern of antibacterial resistance. 55 Clinically affected Holstein Friesian crossbred (20) and Jersey crossbred (35) were studied. Totally 95 isolates were recovered and amongst that Staphylococcus sp. (46.32%), Escherichia coli (27.37%), Streptococcus sp. (12.63%), Klebsiella sp. (9.47%) and Bacillus sp., (4.21%) were documented 40% of isolates yielded more than one bacteria. In-vitro antibiogram revealed that Ceftriaxone (53.47%) was found to be most sensitive followed by Amoxycillin (19.18%), Gentamicin (11.72%). Enrofloxacin (7.89%), Amikacin (4.22%) and Ciprofloxacin (1.22%) were moderately effective. Chloramphenicol (0.77%), Ofloxacin (0.77%) and Tetracycline (0.77%) were least effective. The study suggests that resistance is increasing among bacteria causing mastitis against commonly used antibacterial drugs. Top Keywords Antibiogram, bacteria, cow, in-vitro, mastitis. Top |