Pathological changes in native sheep experimentally infected with bluetongue virus 10 Vineetha S.1,3, Singh K.P.3,*, Maity Madhulina1,3, Sharma G.K.3, Saminathan M.1,3, Sonalika M.2,3, Sahoo Diptimayee1,3, Singh Rohit1,3, Gupta V.K.3 3Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India 1Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India 2Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India *Address for Correspondence K.P. Singh, Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India. e-mail: karam.singh@rediffmail.com
Abstract Bluetongue virus (BTV), the causative agent of bluetongue (BT) in sheep thrives throughout tropical, subtropical and temper-ate regions of the world wherever competent vector population exist for dissemination of the virus. BT has a severe impact on the livestock economy of a country or region. The present study was designed to study the pathology of Indian isolate of bluetongue virus serotype 10 (BTV-10) in experimentally infected native sheep. Six indigenous native sheep were intradermally infected with clarified virus suspension having 106.0 TCID50/ml and four animals were kept as negative control. One animal from each group was sacrificed at 04,07, 11 and 16 DPI and representative organs were collected. Very mild clinical signs were observed in BTV-10 infected animals. Grossly, lymph nodes were foundenlarged, oedematous and hemorrhagic, lungs also oedematous and congested. Microscopically, lymph nodes, spleen, heart, lungs and brain showed mild to moderate lesions in vascular endothelium. Viral antigens could be demonstrated by immunohistochemistry in lymph nodes, spleen and tonsils. BTV antibodies were detected at 5 DPI and the antigens were detected consistently in tongue, thymus, muscle, heart and pulmonary artery at all sacrificed points. The experimental inoculation of BTV-10developedonly subclinical infection in native sheep. How to cite this article: S., Vineetha, Singh, K.P., Maity, M., Sharma, G.K., Saminathan, M., M., Sonalika, Sahoo, D., Singh, R. and Gupta, V.K. Pathological changes in native sheep experimentally infected with bluetongue virus 10 (2019). Indian J. Vet. Pathol., 43(1): 01–06. Top Keywords Bluetongue, Bluetongue virus 10, Experimental, Infection, Pathology, Sheep. Top |