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Year : 2015, Volume : 39, Issue : 1
First page : ( 95) Last page : ( 97)
Print ISSN : 0250-4758. Online ISSN : 0973-970X. Published online : 2015 March 1.
Article DOI : 10.5958/0973-970X.2015.00023.1

Occurrence of aspergillosis in Giriraja chicken from Aizawl, Mizoram

Rajkhowa T.K.2,*, Deka Devajani1,2, Singh Y.D.2

2Department of Veterinary Pathology, College of Veterinary Sciences & Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram-796014

1Veterinary Public Health & Epidemiology, College of Veterinary Sciences & Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram-796014

*Corresponding author: e-mail: tridibraj09@gmail.com

Received:  11  June,  2014; Accepted:  10  January,  2015.

Abstract

An outbreak of respiratory tract affection with failure of antibiotic treatment was investigated in a backyard poultry farm of 100 Giriraja birds in the age group of 6–8 weeks from Aizawl (Mizoram). Twenty five birds showed the clinical signs of respiratory tract affection with increasing severity over a week. Aspergillus fumigatus was isolated and found to be the main etiological agent detected in 18 dead birds. On necropsy, white to yellow caseous nodules were observed in the lungs, air sacs, liver and kidneys. Histological sections of lungs revealed focal granulomatous reaction and thickening of air sac membranes due to heterophil and mononuclear infiltrations. Aspergillus hyphae invading the lung parenchyma were observed in lacto phenol cotton blue stained sections. Aspergillus fumigatus was isolated from the inoculations prepared from the suspensions of organs showing lesions. Therapeutic treatment with copper sulphate orally to the birds and replacement of litter material treated with copper sulphate responded rapidly with decrease in severity in clinical signs and suspended mortality.

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Keywords

Aspergillosis, Giriraja chicken, Mizoram.

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Figures

Fig. 1.:

Multiple cream colored nodules (arrows) distributed throughout the lungs, air sacs, liver and kidneys;




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Fig. 2.:

Lung: mycotic granuloma (arrow) with a central core of necrotic cellular debris and heterophils surrounded by periphery of macrophages, epithelioid cells and aggregates of lymphocytes in the thickened parabronchi of lung parenchyma. H& E ×100;




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Fig. 3.:

Lung: Fungal hyphal invasion in the parabronchi of lung parenchyma. H& E ×200;




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Fig. 4.:

Fungal mycelium with presence of conidiophores, sterigmata and conidia of A. Fumigates in the impression smear. Lacto Phenol Cotton Blue ×100.



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