Diagnosis of group A rotavirus infection in piglets by polymerase chain reaction targeting gene segment 6 Rout M.*,2, Saikumar G.1,2 1Project Directorate on FMD, Mukteswar, Nainital-263138, Uttarakhand 2division of Pathology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India *Corresponding author: email: drmrout@gmail.com
Abstract The present study was conducted during September 2007 to May 2009 to investigate the prevalence of group A rotavirus (GARV) infection in piglets. A total of 199 necropsied intestinal samples and 96 fecal samples from piglets were collected and tested for group A rotavirus with polymerase chain reaction (PCR) targeting gene segment 6. Out of 199 necropsied intestinal samples, 5 (2.51%) samples of piglets within 4 weeks of age and out of 96 fecal samples, 16 (16.66%) samples from piglets within 6 weeks of age were found positive for GARV. The results indicate that group A rotavirus is circulating in certain pig populations of Bareilly, Uttar Pradesh. Top Keywords Faecal samples, Piglets, PCR, Rotavirus. Top |