Year : 2012, Volume : 36, Issue : 1
First page : ( 80) Last page : ( 83)
Print ISSN : 0250-4758. Online ISSN : 0973-970X. Published online : 2012 June 1.

Assessment of effect of antibiotic on intraphagocytic killing in phagocytic somatic cells of clinical mastitis milk

Jha Dilip K.^1,*, Singh K.K.¹, Gupta M.K.¹, Mahto Dinesh¹

¹Department of Veterinary Pathology, College of Veterinary Science & AHBAU, Ranchi-834006 (Jharkhand)

*Corresponding author: email: drdilip20071@gmail.com

Received:  30  January,  2012; Accepted:  30  March,  2012.

Abstract

One hundred forty three mastitis milk samples were collected from organized dairy farms of different districts of Jharkhand. They were treated in vitro with levofloxacin, ceftriaxone, amikacin and ampicillin and thereafter a smear was prepared, dried and subjected to acridine orange staining. Quantitative analysis of effect of different antibacterial agents on bacterial phagocytosis and intracellular killing and degradation was done by acridine orange fluorescence technique. The counting of phagocytic cells in fluorescent microscope depict a true and real picture of interaction, between individual phagocyte and internalized organism, going on in milk in presence of antibiotics. From the present study it can be said that ceftriaxone and amikacin could prove to be a better medicine for parentral application due to their higher bactericidal and poor leucocidal property. Levofloxacin on the other hand will be better choice for intra mammary use because of its better leucocidal and bactericidal property. However, there may be variation in this trend in individual cases of mastitis whose assay will be helpful in appropriate selection of antibiotics.

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Keywords

Amikacin, Ceftriaxone, Levofloxacin, Mastitis, Phagocytosis.

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