Ameliorative efficacy of Ocimum sanctum leaf extract in lead acetate induced toxicity in Wistar albino rats: A pathomorphological study Sujatha K.*,3, Srilatha Ch.3, Anjaneyulu Y.3, Rao T.S. Chandrasekhar1,3, Sreenivasulu D.2,3, Amaravathi P.3 3Department of Pathology, College of Veterinary Science, Sri Venkateswara Veterinary UniversityTirupati-517502 2Department of Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary UniversityTirupati-517502 1department of Pathology, College of Veterinary science, Rajendra Nagar, Hyderabad (A.P.) *Corresponding author: email: karamalasujatha@gmail.com
Abstract In the present investigation, 216 healthy adult rats were randomly divided into six groups viz. control (group I), higher dose of lead acetate @60mg/kg bwt (group II), lower dose of lead acetate @ 30 mg/kg bwt (group III), higher dose of lead + Ocimum @ 400 mg/kg bwt (group IV), lower dose of lead + Ocimum @ 400 mg/kg bwt (group V), Ocimum control (group VI). All lead treated and ameliorated groups given lead acetate/lead + Ocimum orally for three days in a week for a period of twelve weeks and histopathological changes were studied in different organs. Dose dependent changes were noticed in all the organs. Liver revealed congestion, degenerative changes, moderate to severe fatty changes, proliferation of bile ducts and microgranuloma in all lead treated groups. In kidneys, degenerative changes, intertubular haemorrhages, atrophied and cystic glomeruli were observed in majority of higher doses of lead treated rats. Lungs of higher doses of lead treated groups revealed moderate to severe congestion, edema, perivascular and peribronchiolar infiltration of mononuclear cells. Histopathologically, cerebrum revealed congestion, submeningeal haemorrhages, degenerative changes in neurons, gliosis and demyelinating changes in all lead treated groups. Moderate to severe depletion of germinal centers were noticed in the spleen and lymph nodes. Based on the changes, it was concluded that lead acetate at 60 mg/Kg b.wt. and 30 mg/Kg b.wt. for 12 weeks was toxic to rats and ameliorative effect of OS was moderate in higher lead dose group and amelioration was effectively observed in lower dose levels of lead acetate. Top Keywords Amelioration, Lead acetate, Ocimum sanctum, Pathomorphology, Wistar rats. Top |