Use of immuno-compromised mouse model for establishment and study of human animal tumours Ingle A.D.*,2, Hosetti B.B.1,2 2Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre (TMC), Kharghar, Navi Mumbai-410 210, MS 1Dept. of Applied Zoology, Kuvempu University, Karnataka-577 451 *Corresponding author; e-mail: aingle@actrec.gov.in; basaling@yahoo.co.in
Abstract Use of immuno-compromised animal models for transplantation of human/animal tumour xenografts has opened a new era for studying tumour pathogenesis. Human/animal tumour xeno-transplantation in immuno-compromised mice before establishment of cell lines enables generation of more novel human cell lines for investigations. Keeping in view of this fact, the present study was undertaken to establish the fresh human tumour xenografts in to the NOD SCID mice. Fresh human tumours collected by the Biorepository Laboratory from the operation theater of the Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), were obtained for this study and implanted surgically under subcutis of the NOD SCID mice. Tumours tissues obtained from oral, thigh, breast and brain were successfully grown in NOD SCID mice. After the immuno-compromised mice have accepted the surgically implanted fresh tumour pieces, these animals were sacrificed at the stage when the tumours attained minimum size of 20 mm of diameter. Part of this tumour was passaged in another fresh animals and remaining part was stored in liquid nitrogen for future use. Human origin of such tumours grown in the mice was ascertained by PCR based analysis of the tumour DNA using STR method. This way original human tumour was established in NOD SCID mice and frozen down without changing the histomorphology which could be made available to the researchers repeatedly and for a long time without altering the genotype as well as without formation of cell lines. Top Keywords Human, NOD SCID mice, STR, Subcutaneous, Tumour, Xenograft. Top |