Hepatotoxicity due to diclofenac alone and under the influence of certain variables in broilers Haritha C.2, Reddy A. Gopala2,*, Anjaneyulu Y.1,2, Kalakumar B.2 2Department of Pharmacology & Toxicology; College of Veterinary Science, Rajendranagar, Hyderabad-500 030 (AP), India 11Dept of Pathology, College of Veterinary Science, Rajendranagar, Hyderabad-500 030 (AP), India *Corresponding author Email: gopalareddy123@rediffmail.com
Abstract Diclofenac toxicity was studied in male broiler chicks (Cobb strain) of a day old age. The chicks were randomly divided into eight groups consisting of ten in each group. Group 1 was kept as basal diet control (1–32 days), group 2 on basal diet for 32 days + diclofenac (0.8 mg/kg body weight I/M) on day 24, 26, 28, 30 and 32, group 3 on basal diet for 32 days + cyclophosphamide (50 mg/kg body weight I/M once daily) from day 20 to 23, group 4 on high protein, high calcium, low vitamin A (HPHC) diet (1–32 days), group 5 on basal diet+ diclofenac + cyclophosphamide, group 6 on HPHC + diclofenac, group 7 on HPHC + cyclophosphamide and group 8 on HPHC + diclofenac+ cyclophosphamide. The concentration of GGT, ALT, total proteins and globulins (blood) revealed significant (P < 0.05) increase, while there was a significant (P < 0.05) decrease in the concentration of albumin, A/G ratio in the groups given diclofenac either alone or in combination with other variables. The histopathology of liver, revealed lesions of mild to marked severity in different combinations. It can be concluded that diclofenac has the hepatotoxic potential in poultry at sub-therapeutic doses and further the toxic effects are more pronounced under the influence of immunosuppressants and HPHC diet. Top Keywords Diclofenac, Hepatotoxicity, Oxidative stress. Top |