Single strand conformation polymorphism at growth hormone gene in Jamunapari breed of goat Vyas M.K., Dixit S.P., Kumar S., Wickramaratne S.H.G., Kaur Jagdeep, Singh N.P., Singh M.K. Cental Institute for Research on Goats, Makhdoom, Farah, Mathura, India. National Bureau of Animal Genetic Resources, Karnal, 132 001, India. Punjab University, Chandigarh, India. Abstract The genomic DNA was isolated from 140 blood samples of Jamunapari flock maintained at Central Institute for Research on Goats, Makhdoom. Three fragments of growth hormone gene (gGH) having both the intronic and exonic regions were amplified by PCR. The PCR-SSCP protocol was standardized to detect genetic variants at gGH in this breed of goat. SSCP analysis of fragment from 1011 to 1344, 1401 to 1604 and from 1705 to 2093 bp of gGH gene revealed 7, 5 and 4 electrophoretic patterns, respectively. The preliminary results showed that SSCP analysis is a valuable tool for the establishment of allelic variants in the genes that express hormones associated with production traits. Top Key words gGH gene, Goat, Jamunapari, Single Strand Conformation Polymorphism. Top |