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Year : 2023, Volume : 36, Issue : 3
First page : ( 442) Last page : ( 448)
Print ISSN : 0971-8184. Online ISSN : 0976-1926. Published online : 2023 December 21.

Genetic diversity analysis of bitter gourd (Momordica charantia L.) germplasm using molecular markers

Adarsh Anupam1, Kumar Pankaj2, Prasad Bishun D.2, Kumar Randhir3, Singh Hemant K.4,*

1Krishi Vigyan Kendra, Saraiya, Muzzafarpur, Bihar, India

2Department of Molecular Biology & Genetic Engineering, Bihar Agricultural University, Bhagalpur, Bihar, India

3Department of Horticulture (Vegetables & Floriculture), Bihar Agricultural University, Bhagalpur, Bihar, India

4Farm Science Centre, Kishanganj, Bihar, India

*Author For Correspondence: horthemant16@gmail.com

Online Published on 21 February, 2024.

Received:  10  August,  2023; :  05  September,  2023; Accepted:  05  September,  2023.

Abstract

Bitter gourd [Momordica charantia L.] is an important cucurbitaceous crop of medicinal and nutraceutical value. The present study was conducted to compare the efficacy of random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) molecular markers for genetic diversity analysis of bitter gourd. Twenty-one genotypes of bitter gourd, collected from different parts of India, were used for diversity analysis using two different types of markers. RAPD and ISSR markers yielded an average of 7.16 and 5.73 amplicon per primer, respectively. In the present study, RAPD and ISSR showed 89 and 63% polymorphic bands, respectively. Combined data analysis of RAPD and ISSR markers provided 73.66% polymorphism with 5.33 polymorphic amplicons per primer. The better discriminatory power of ISSR markers over RAPD markers may be due to comparatively higher values of average polymorphic information content (0.175) as well as the diverse nature of the genotypes. The information generated in this study would be helpful in designing breeding strategies for bitter gourd improvement.

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Keywords

Bitter gourd, Cluster, Genetic diversity, Inter simple sequence repeats, Random amplified polymorphic DNA.

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