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Year : 2023, Volume : 36, Issue : 3
First page : ( 396) Last page : ( 401)
Print ISSN : 0971-8184. Online ISSN : 0976-1926. Published online : 2023 December 21.

Development of cryoconservation protocol for In-vitro shoot bases of Allium ampeloprasum L. for long-term conservation

Gowthami Ravi1, Chander§ Subhash1, Pandey Ruchira1, Shankar Muthusamy2, Agrawal Anuradha1,3,*

1Tissue Culture & Cryopreservation Unit, ICAR-National Bureau of Plant Genetic Resources (NBPGR), New Delhi, India

2Division of Plant Genetic Resources, ICAR-Indian Agricultural Research Institute, New Delhi, India

3National Agricultural Higher Education Project (NAHEP), Krishi Anusandhan Bhawan-II, Indian Council of Agricultural Research (ICAR), Pusa Campus, New Delhi, India

*Author For Correspondence: anuradha.agrawal@icar.gov.in

Online Published on 21 February, 2024.

Received:  29  March,  2023; :  25  May,  2023; Accepted:  26  May,  2023.

Abstract

Critical factors for the development of cryo conservation protocol for Allium ampeloprasum L. conserved in the in-vitro Genebank (IVGB) at ICAR-NBPGR of India were investigated. Shoot bases (1.0 × 1.5 mm) excised from 8-wk-old shoot cultures grown on growth media (MS + 0.1 mg/l NAA + 0.02 mg/l 2iP + 0.3 M sucrose) and pregrown at 22/ 5°C for 16/8 hours alternate temperature regime, precultured on MS medium supplemented with 0.3 M sucrose at standard culture conditions (SCC) for 16 hours followed by loading solution treatment for 60 minutes, PVS2 dehydration for 40 minutes were successfully cryoconserved using vitrification and droplet-vitrification technique. Droplet-vitrification technique improved post-thaw regrowth by ~58.33% as compared to vitrification. Thus, a standardized protocol was used for cryobanking of shoot bases of A. ampeloprasum.

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Keywords

Allium ampeloprasum, Vitrification, Droplet-vitrification, Shoot bases, Cryobanking.

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