Molecular characterization of wheat seed gall nematode, Anguina tritici Gawade Bharat H.*, Priyanka1, Khan Zakaullah, Kumar Pardeep, Chalam V. Celia Division of Plant Quarantine; ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012, India 1Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Science, Prayagraj-211007, Uttar Pradesh, India *Corresponding author; E-mail: bharat.iari@gmail.com, bharat.gawade@icar.gov.in
Online Published on 29 June, 2022. Abstract Wheat seed gall nematode, Anguina tritici is one of the important nematodes of wheat (Triticum aestivum L.), distributed in the major wheat growing areas of the world. It is the first plant parasitic nematode described by Needham in 1743. In India, A. tritici is the only Anguina species reported, however, its molecular characterization is lacking. In addition, there is need to study taxonomic similarity of Indian population of A. tritici with its other populations andspecies under related genera. The present study was focused on molecular characterization of A. tritici using gene sequences of different conserved regions such as Internal Transcribed Region (ITS), D2-D3 expansion region of 28s rRNA and phylogeny studies. Gene sequences of both the regions were PCR amplified using universal primers and corresponding size of ITS and D2-D3 region of 28s rRNA was found ~950 bp and ~750 bp, respectively. Both ITS and 28s rRNA gene products were sequenced and found identical to A. tritici populations (>99% similarity). Phylogeny trees were used to ascertain the relationships of it with other nematodes of Anguinidae family and revealed that A. tritici population studied was different from other seed gall forming and aerial nematodes, however, it was similar to other A. tritici populations. Top Keywords 28s rRNA, Anguina tritici, Internal Transcribed Region, Molecular characterization. Top |