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International Journal of Medical Research & Health Sciences
Year : 2012, Volume : 1, Issue : 1
First page : ( 1) Last page : ( 6)
Online ISSN : 2319-5886.

An experimental study on effect of antioxidant vitamin E in stress and alcohol induced changes in male fertility in albino rats

Sanghishetti Vijay Prasad1,*, Ghongane BB2, Nayak BB1, Raul AR1, Kumar AN Vijay1, Mutalik MM1, Kapure NL1

1Department of Pharmacology, Padmashree Dr Vitthalrao Vikhe Pail Foundation's Medical College, Ahmednagar, Maharashtra

2Department of Pharmacology, Govt. Medical College Miraj, Maharashtra

*Corresponding author: Email: vijayfarmac@gmail.com

Online published on 6 April, 2013.



Physical and Psychological stresses are believed to reduce sexual functions, resulting from neurotransmission changes in various erectile response pathways and reduced blood flow in genital organs. Intake of alcohol depends on numerous genetic and environmental factors. Stress has long been thought to influence the alcohol drinking in humans. Forced swimming in laboratory animals has been widely used as a model of stress to study the physiological changes and the capacity of the organism in response to stress.


1. To assess the effect of forced swimming stress on seminal fluid profile, Serum testosterone level, Testicular lipid peroxidation levels. 2. To assess the effect of Vitamin E on stress induced changes.


Adult male albino rats weighing 200 – 220 g, aged 12–15 weeks were used in this study. The animals were randomly divided into four groups of 6 animals each. Group1 (control) received distilled water, Group 2 (Forced Swimming Stress) received distilled water Group 3: subjected to Forced Swimming stress with 20% alcohol intake p.o. Group 4: Swimming stress with 20% alcohol treated with Vitamin E (200mg/kg/day orally). The following parameters were studied in all groups 1. Body weight. 2. Sperm count Motility and Life/death Ratio (SLDR). 3. Serum Testosterone 4. Testicular Malondialdehyde level (MDA).


Forced swimming stress caused loss in body wt, reduction in sperm count, motility and SLDR in sperm analysis, reduction in serum testosterone and increase testicular MDA levels compared to control. All the changes were statistically significant. When alcohol was added along with forced swimming it caused further loss in body wt, sperm count, motility and SLDR, serum testosterone level and slightly further increase in MDA levels. These observations were also statistically significant. In case of group IV in which Vitamin E was administered long with stress with alcohol it showed a trend of reversal phenomenon of stress and alcohol induced change (Group III) exhibited by gain in body wt, increase sperm count, motility and SLDR, serum testosterone and decrease in MDA level. All the results were statically significant except serum testosterone.


Force swimming indicates that swimming is an effective model for producing stress in albino rats. The stress induced changes were further accentuated by addition of ethyl alcohol. However Vit E treatment reverses the effect of stress and alcohol.



Forced swimming stress, Alcohol, Vitamin E, Male fertility.


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