Indian Journal of Horticulture
Year : 2000, Volume : 57, Issue : 3
First page : ( 228) Last page : ( 230)
Print ISSN : 0972-8538.

Effect of yeast and malt extracts on embryogenesis in Citrus species

Parthasarathy V.A.*, Nagaraju V.

Division of Horticulture, ICAR Research Complex for NEH Region, Umiam (Barapani) 793 103, Shillong, Meghalaya

* Present address: CPCRI, Kasaragod 671 124, Kerala

Summary

In-vitro culture studies were conducted with excised ovules (8–10 weeks old) of Citrus species, viz. Khasi mandarin (Citrus reticulata Blanco), Cleopatra mandarin (Citrus reshni Tanaka) and Calamondin (Citrus madurensis Lour) in Murashige and Skoog's medium supplemented with yeast and malt extracts (500 mg/litre each). Observations recorded 12 weeks after culture initiation showed differential responses of the organic supplements used. Malt extract was superior as compared to yeast extract in inducing more number of cotyle-donary, torpedo and globular embryoids. It was observed that abnormal embryoids formation occurred in Khasi mandarin and cluster of undifferentiated embryogenic callus occurred in Cleopatra mandarin.

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