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Analysis of Antigen Sharing Amongst Gastro-Intestinal Nematodes of Sheepand Goats Using Counter Immuno Electrophoresis Arunkumar S., Sharma R.L. Department of Veterinary Parasitology, Madras Veterinary CollegeTamil Nadu Veterinary and Animal Sciences University, Chennai - 600 007. Abstract Antigen sharing amongst gastro-intestinal nematodes viz., Haemonchus contortus, Oesophagostomum columbianum and Bunostomum trigonocephalum was evaluated by counter immuno electrophoresis. Two antigens namely soluble extract antigen (SEA) and gut integral membrane antigen (GIMA) were probed with rabbit hyper immune sera raised against SEA of three referral nematodes to discern out the image of identity. The immunoreactivity pattern of referral nematode antigens showed generally 2–3 precipitation lines with homologous and 1 precipitation line with heterologous sera. Evidently these results suggested the existence of cross-reacting antigenic determinants amongst the SEA and GIMA of referral nematodes. Top Keywords Antigen sharing, nematodes, counter immuno electrophoresis. Top | |
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