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Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 4
First page : ( 1078) Last page : ( 1084)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00443.2

The Arg399Gln (rs25487) Polymorphism of XRCC1 Gene as a Predictor for Uterine Leiomyoma in Women of Babylon Province/Iraq

Tajuldeen Lamees Talib1, Alshalah Haydar Hashim2, Al-Hilli Nadia Mudher3

1M.B. Ch. B., M. Sc. Clinical Biochemistry, Ministry of health, Babylon health directorate, Hilla, Iraq

2F.I.C.M.S. Chemical Pathology, University of Babylon, College of Medicine, Hilla, Iraq

3F.I.C.O.G. Obstetrics & Gynecology, University of Babylon, College of Medicine, Hilla, Iraq

Online published on 27 November, 2019.

Abstract

Uterine leiomyoma (UL) is a benign monoclonal smooth muscle tumour. The exact cause for this benign tumour is unknown. Evidences that are available from previous studies are not certain but are consistent in pointing to an association or a heritable etiology for the involvement of single nucleotide polymorphism (SNP) for the development of uterine leiomyoma. Aim of study: To reveal if there is an association between XRCC1 gene polymorphism Arg399Gln (rs25487) and uterine leiomyoma development in Iraqi Arabian women from Babylon province in comparison to control group. This case-control study enrolled 238 white Iraqi Arabian subjects, 118 women with uterine leiomyoma (cases) and 120 (controls). Genotyping of XRCC1gene (rs25487) single nucleotide polymorphisms were carried out by PCR-RFLP. MspI was used for XRCC1 gene product followed by electrophoresis on agarose gel. Various statistical analyses were applied to analyze the data. XRCC1gene (rs25487) polymorphism revealed significant positive association with uterine leiomyoma development in Iraqi Arabian women, women with recessive GG genotype in comparison to (AG+AA), may be seven times more susceptible to uterine leiomyoma development, (odd ratio =7.59, C.I.95% = 3.77–15.25, p-value <0.0001

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Keywords

Uterine leiomyoma, XRCC1 gene, Arg399Gln polymorphism.

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