Indian Journal of Ecology
Year : 2021, Volume : 48, Issue : 1
First page : ( 123) Last page : ( 127)
Print ISSN : 0304-5250.

Comparative analysis and characterization of lipase producing alkaliphilic bacteria isolated from Lonar Soda Lake, India

Dhundale Vishal R.*

New Arts Commerce and Science College, Ahmednagar-414 001, India

*E-mail:vrdvishal@gmail.com

Online published on 9 March, 2021.

Abstract

Microbial culture dependent phenotypic and genomic characteristic were observed for lipase producing bacteria. Out of twenty nine isolates; thirteen bacterial strains were obtained lipase producing bacterial strains. These strains were subjected to physiological and standard biochemical characterization along with 16S rDNAsequencing for their identification. The phylogenetic position indicated the bacterial strains were related to phylum Firmicutes and belongs to Bacillus cereus OCW31, Bacillus pumilus DW21, Bacillus pseudofirmus DW41 and Bacillus flexus AW32. These four strain selected for production and, partial characterizations of lipase due to maximum lipolytic activity. The lipase was highly stable over a broad temperature from 40 to 90°C and optimum enzyme activity was found to be 70°C for B. cereus ICW31 . The effect of organic solvents on the lipase activity was determined. The data elucidate that the lipase from B. flexus AW32 and B. pseudofirmus DW41 was highly active than B. cereus OCW31 and B. pumilus DW21 in all organic solvents tested. The lipase was enhanced the activity in presence of CaCl₂ MnSo₄, for B. pseudofirmus DW41 and B. pumilus DW21 while Nicl₂ enhanced the lipase activity produce by B. pseudofirmusDW41 and B. cereus OCW31. The information for enzyme production and optimization of lipase has a bright future towards the improvement and production of novel enzymes for biotechnological potential.

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Keywords

Lonarlake, Lipase, Alkaliphilic bacteria, Bacillus.

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