Identification and Characterization of Mosquitocidal Toxins from Bacillus cereus VCRC-641 Manikandan S1, Aneha K1, Bora Bhagyashree1, Abhisubesh V1, Hemaladkshmi P1, Gangmei Kakhuangailiu1, Mandodan Sahadiya1, Lukose Jibi1, Mathivanan A1, Vijayalakshmi K1, Poopathi S1,* 1Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry605006, India *Email: subbiahpoopathi@rediffmail.com (corresponding author): ORCID ID 0000-0002-2366-3689
Online Published on 31 August, 2024. Abstract Bacillus cereus VCRC 641 was found to be an alternate larvicidal bacterial agent which was newly isolated from fresh water fish, Clarias batrachus (walking cat fish). The protein responsible for mosquito toxic effect was separated and purified by Sephacryl S-200 column chromatography and NATIVE PAGE. It was observed that a unique protein with molecular weight of 70kDa was the responsible factor for mosquito killing effect. Further analysis of the protein by liquid chromatography-mass spectrometry (LC-MS) it was identified as “Uncharacterized Protein”. The length of the uncharacterized protein was around 158bp. The uncharacterized protein was expected to have a molecular mass of 70 kDa, 51 amino acids, confidence score of 78.45 and mass of 206772.57. Therefore, in the present study we report for the first time that uncharacterized protein (70kDa) was the responsible factor for the mosquito killing effect. Top Keywords Bacillus cereus, Native page, LC-MS, SDS-PAGE, Uncharacterized protein, Amino acids, Trypsin, Mosquitocidal, Toxins, Vector control, Bio assay, Clarias batrachus. Top |