Proteomic Analysis of Cowpea Aphid Aphis craccivora Koch Salivary Gland using LC-MS/MS Analysis Pavithran S1, Murugan M1,*, Jayakanthan M2, Balasubramani V1, Harish S3, Senthil N2 1Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore641003, Tamil Nadu, India 2Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore641003, Tamil Nadu, India 3Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore641003, Tamil Nadu, India *Email: muruganmarimuthu@tnau.ac.in (corresponding author): ORCID ID 0000-0002-7485-1153
Online Published on 31 August, 2024. Abstract The present study identified 151 proteins from the salivary gland of cowpea aphid Aphis craccivora Koch, using LC-MS/MS analysis. These included enzymes mainly involved in the digestion and detoxification of secondary metabolites and proteins related to cell development and molecular function. Enzymes like peroxidase, trehalase, cytochrome P450 monooxygenase, glutathione peroxidase, esterase, peptidase, carboxypeptidase, maltase, and beta-galactosidase were prevalent in the proteome. Additionally, several proteins were assigned to cellular and molecular functions of salivary gland. These proteins may be involved in host-plant interactions. Comprehensively, these results provide a database for elucidating aphid-plant interactions at the molecular level in the future. Top Keywords Aphis craccivora, Vigna radiata, Salivary gland, Protein, LC-MS/MS, Digestion, Detoxification enzymes, Functional annotation, Gland dissection, Proteomic analysis. Top |