Estimation of allelic frequency of -Casein and -Lactoglobulin genes in Bos Indicus cattle breeds Dadhich Shubha*, Patel RK**, Soni KJ**, Singh KM**, Chauhan JB** Banasthali Vidyapith, Rajasthan. *Department of Bioscience and Biotechnology.
**R&D-Biotechnology, National Dairy Development Board, Anand388 001 Email: rkpatel46@yahoo.com
Abstract A polymerase chain reaction-restriction fragment length polymorphism test was performed on DNA extracted from blood samples of Bos indicus cattle breeds to estimate allelic frequency of the bovine kappa casein and beta-lactoglobulin gene responsible for milk production traits. A 350 bp fragment of kappa casein and 247 bp fragment for beta-lactoglobulin were amplified and digested with Hinf I and Hae III restriction enzymes, respectively. Two types of alleles A and B and three types of genotypes AA, BB and AB for kappa casein and beta-lactoglobulin were observed. In present study, the allelic frequency for B allele was found double (0.662) than A allele (0.338) for beta-lactoglobulin. Similarly allelic frequency for A allele was three and half times more than B allele for kappa-casein in Bos indicus cattle population. These genotypes are useful markers for milk production traits on which bull can be evaluated and selected for future breeding programmes. Top Key words Allele, Bos indicus, κ-Casein, β- Lactoglobulin, PCR-RFLP, Genotype. Top |