Physico-Biochemical attributes of semen and their interrelationships in triple crossbred (HF X J X K) bulls Raval Rupesh J, Dhami AJ Anand Agricultural University, Anand-388 001, India. *LRS, College of Veterinary Science and Animal Husbandry, E-mail: dhami_1659@yahoo.com
Abstract Semen ejaculates (32) of 4 mature triplebred (25% HF x 25% J x 50% Kankrej) bulls collected at weekly interval during winter were used to study seminal characteristics as well as seminal plasma biochemical and enzymatic profile and their interrelationships. The mean ejaculate volume, mass activity (score 0–5), sperm concentration, individual sperm motility, live sperm and abnormal sperm recorded were 3.88±0.16 ml, 3.73±0.06, 999.06±16.14 million/ml, 84.00±1.02%, 86.28±0.97% and 8.28±0.44%, respectively. The average seminal plasma content of GOT, GPT, AKP, total protein, total cholesterol, calcium, inorganic phosphorus and magnesium was 189.11±6.29 IU/L, 82.53±4.29 IU/L, 718.94±24.27 IU/L, 6.95±0.34 g/dl, 42.77±2.87 mg/dl, 21.26±0.56 mg/dl, 44.16±1.30 mg/dl and 2.89±0.06 mEq/L, respectively. The bulls varied significantly (P<0.05) in their ejaculate volume, sperm concentration, abnormal sperm per cent, seminal plasma total protein, total cholesterol, GPT, calcium and magnesium levels. Moreover, the ejaculate volume had significant positive correlation (P<0.01) with abnormal spermatozoa (r=0.41); mass activity with the initial motility (r=0.81), abnormal sperm (r=-0.46) and plasma total protein (r=0.50); live sperm percentage with initial motility (r=0.54), abnormal sperm (r=-0.60) and plasma cholesterol (r=0.36); initial motility with abnormal sperm (r=-0.59) and plasma GPT (r=0.34) and sperm concentration with seminal plasma GPT, total protein and magnesium levels (r=-0.43, -0.46, -0.39). Seminal plasma GOT activity was significantly (P<0.01) correlated with plasma GPT, total protein, total cholesterol and magnesium concentrations (r=0.46, 0.39, 0.36, 0.39, resp), while GPT activity had significant correlations only with plasma protein and magnesium contents (r=0.64, 0.60), and magnesium with total protein and calcium levels (r=0.65, 0.39). Plasma AKP and inorganic phosphorus levels did not show significant correlations with any of the physico-biochemical attributes studied. Semen quality of all 4 triplebred bulls was optimum and its’ biochemical profile was within normal physiological limit. Top Key words Semen characteristics, Seminal plasma, Biochemical profile, Triple crossbred bulls, Correlations. Top |