Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2010, Volume : 31, Issue : 1and2
First page : ( 33) Last page : ( 36)
Print ISSN : 0970-9320. Online ISSN : 0974-0147.

Plasmid characterization and curing study of Listeria species isolated from foods of animal origin

Jaulkar A.D., Zade N.N., Katre D.D., Chaudhari S.P., Khan W.A., Shinde S.V.

Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra Animal and Fishery Science University, Seminary Hills, Nagpur-440006, (Maharashtra). drashwini_23@rediffmail.com

Abstract

Nine isolates of Listeria species from the foods of animal origin were processed for plasmid profile studies, using the techniques of alkaline lysis and agarose gel electrophoresis. Plasmid profiling revealed seventeen plasmid profile patterns with molecular weight ranging from 27.642 kb to 360.850 kb. Curing study of seven azithromycin resistant plasmid bearing listerial isolates responded to curing by showing elimination of plasmids. The frequency of curing was found to be 100 per cent. Processing of the cured isolates for further antibiotic sensitivity against azithromycin demonstrated sensitivity thus revealing correlation among plasmid bearing and antibiotic resistance. Findings of the present study indicate the possibilities of plasmid profile as a valuable epidemiological tool in differentiating the strains with different source of origin.

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