Antibiogram study and plasmid characterization of Aeromonas species from foods of animal origin Jaulkar A.D.1, Prof. & Head, Zade N.N.2, M.V.Sc. Student, Katre D.D.3, Assoc. Prof., Chaudhari S.P.4, Assoc. Prof., Shinde S.V.4, Assoc. Prof., Khan W.A.*, M.V.Sc. Student Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra Animal and Fishery Science University, Seminary Hills, Nagpur-440006, (Maharashtra) 1Dept. of Veterinary Public Health, Nagpur Veterinary College, Nagpur 2Dept. of Veterinary Public Health, Nagpur Veterinary College, Nagpur 3Dept. of Veterinary Public Health, Nagpur Veterinary College, Nagpur 4Dept. of Veterinary Public Health, Nagpur Veterinary College, Nagpur * Corresponding author: Dept. of Veterinary Public Health, Nagpur Veterinary College, Nagpur: drashwini_23@rediffmail.com
Abstract Fifteen isolates of Aeromonas species were studied for antibiotic sensitivity, plasmid isolation, curing and correlation of plasmids with phenotypic expression viz. antibiotic resistance. Antibiogram study revealed that 93.33% isolates were resistant to penicillin-G followed by amoxyclav and tetracycline (80% each), ampicillin (73.33%), nalidixic acid (60%), doxycycline hydrochloride (46.66%), trimethoprim (40%), azithromycin, ceftazidime and neomycin (20% each), and the least resistance was observed to cephotaxime, erythromycin, gentamicin and streptomycin (6.66% each). Moderate degree of sensitivity was noticed towards erythromycin (73.33%) followed by trimethoprim (26.66%), ceftazidime and cephotaxim (20% each), amoxyclav, amikacin, doxycycline hydrochloride, neomycin, nalidixic acid and streptomycin (13.33% each) and to ampicillin and penicillin-G by 6.66% isolates. Isolates (93.33%) were sensitive to gentamicin, amikacin (86.66%), azithromycin and streptomycin (80% each), cephotaxim (73.33%), neomycin (66.66%), ceftazidime (60%), doxycycline hydrochloride (40%), trimethoprim (33.33%), nalidixic acid (26.66%), ampicillin, erythromycin and tetracycline (20% each) and least sensitive to amoxyclav (6.66%). Eleven 11 isolates harboured one to two plasmid fragments with molecular weight ranging between 29.321 kb to 274.315 kb. Nine plasmid bearing and tetracycline resistant isolates subjected to curing study using potassium nitrate as a curing agent, revealed elimination of plasmid of molecular size of 81.230 kb and 66.530 kb from two isolates. The frequency of curing was found to be cent per cent. Processing of the cured isolates for further antibiogram study against tetracycline revealed sensitivity indicating plasmid mediated tetracycline resistance. Top |