Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2010, Volume : 31, Issue : 1and2
First page : ( 1) Last page : ( 4)
Print ISSN : 0970-9320. Online ISSN : 0974-0147.

Induction of TLR-3 expression in IBD virus challenged chickens from genetically different background

Kumar B.S. Ananda, Dandapat S., Kumar P., Kumar S., Principal Scientist & Incharge, CIF, Ram G.C., Emeritus Scientist

Immunology Section, Indian Veterinary Research Institute, Izatnagar-243122, (Uttar Pradesh). satyadandapat2008@hotmail.com

Abstract

The present study was designed to study the role of TLR-3 in relative disease resistance to IBD virus infection in the indigenous Kadaknath and White-leghorn chicken. Macrophages and splenocytes isolated from IBD virus challenged and Poly I:C inoculated birds of both the breeds were analyzed for TLR-3 expression pattern. Macrophages from IBD challenged Kadaknath breed showed maximum of 3.59 fold increase in TLR-3 expression at 24^th hr interval and moderate expression of 2.17 fold at 12^th hr interval as compared to white leghorn. In case of splenocytes, IBD challenged Kadaknath breed showed maximum of 20.11 fold increase in TLR-3 expression at 24^th hr of interval and moderate expression of 5.37 fold at 12^th hr interval. Between the breeds the IBD virus induced TLR-3 expression was more in Kadaknath as compared to White leghorn. In Kadaknath breed the poly I: C could induce only 5 and 2 fold increase of TLR-3 expression in splenocytes at the same intervals as compared to IBD virus, showing natural infection can induce more of TLR-3 expression than synthetic analogs.

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