Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2004, Volume : 25, Issue : 1
First page : ( 33) Last page : ( 35)
Print ISSN : 0790-9320.

Characterization of Stimulator cells in the Caprine Primary Mixed Lympocyte Reaction and Oxidative Mitogenesis

Ananda Chitra M.², Ram G.C.¹, Goswami T.K.¹

¹Immunology Section Indian Veterinary Research Institute Izatnagar - 243 122 (U.P.)

²Veterinary Assistant Surgeon, CB&FD, Sivagangai, Tamilnadu

Primary mixed leucocyte reaction and oxidative mitogenesis are the two most common and reliable assay systems used to demonstrate the stimulating capacity of antigen presenting cells. Mitomycin C treated and not treated macrophages (MF) and B cells were used as stimulator to the T cells which were treated with sodium periodate (oxidative mitogenesis) or without treatment (Mixed leucocyte reaction, MLR). The magnitude of proliferation associated with MF and B cell function in oxidative mitogenesis was at least 2-fold higher than MLR. Both B cells and MF did not vary much in their ability as accessory cells in MLR as well as in oxidative mitogenesis. Mitomycin C treated or untreated stimulator cells had similar effect.

Top