Effect of TRIM28 on proliferation, apoptosis and histone H3K9 trimethylation in bovine fibroblasts Panpan Ma1,, Man Xiaxia2,, Yang Shubao1, Dong Xiaoqing1, Liyan Su1, Luan Weimin1,*, Ma Xin1,* College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China 1College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130 118, China 2Department of Oncologic Gynecology, The First Hospital of Jilin University, Changchun, 130021, Jilin, People's Republic of China *Corresponding author's e-mail: maxin3202@163.com
Abstract TRIM28 is a co-repressor, which interacts with HP1 proteins and chromatin repressive complexes leading to gene expression silencing. In this study, after we used Lipofectamine3000-mediating siRNA and restructured p EGFP-IRES2-TRIM28 to transfect bovine fibroblasts, the expression levels of TRIM28, HP1BP3, DNMT, SETDB1, TP53, BAX, Bcl and SOD were detected by real-time polymerase chain reaction (PCR). In addition, the influence of p EGFP-IRES2-TRIM28 on histone H3K9me3 was also observed. In the siRNA transfected group, TRIM28 was down-regulated (P<0.01), which inhibited cell proliferation and reduced the level of H3K9me3 expression. In the p EGFP-IRES2-TRIM28 transfected group, TRIM28 was over-expressed (P<0.01), and the pro-apoptosis gene BAX was significantly decreased (P<0.01), but there was no significant change in other genes. Our results demonstrate that TRIM28 plays an important role in bovine fibroblasts and might be a valuable biomolecule for proliferation, apoptosis, and histone H3K9 trimethylation. Top Keywords Bovine fibroblasts, Down-regulation, Over-expression, TRIM28. Top |