Indian Journal of Animal Research
Year : 2007, Volume : 41, Issue : 1
First page : ( 1) Last page : ( 8)
Print ISSN : 0367-6722.

Studies on the genotoxic effects of sucralose in laboratory mice

Sharma Ashwani¹, Panwar Sunil¹, Singh A.K.¹, Jakhar K.K.^1,2

¹National Dairy Research Institute, Karnal-132 001 Haryana, India.

²Veterinary Diagnostic Lab, CCS HAU, Hisar.

Abstract

Forty female (Swiss albino) mice were divided into 4 groups of ten animals each. Mice of groups I, II and III were fed sucralose 500, 1000 and 1500 ppm, respectively in feed. Group IV was fed sucralose free diet. Five animals were sacrificed from each group on 30^th and 60^th day of study and metaphase spreads prepared for evaluation of mitotic index and chromosomal aberrations. Liver and kidney samples of mice were also collected on 30^th and 60^th day of experiment for histopathological study. Among the chromosomal aberrations observed, polyploidy and monosomy were most common. The differences in mitotic indices and chromosomal aberrations between the 4 groups at different intervals were found to be statistically non-significant. Histopathological changes were observed in the liver and kidney of all treated groups. It is concluded from the present study that sucralose consumption up to 1500 ppm for two months did not produce any genotoxicity but it is hepatotoxic and nephrotoxic. However, its effect at higher doses and longer duration needs to be studies.

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