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Evaluation of nuclear maturation in Black Bengal goat oocytes cultured in vitro Pampori Z.A.1, Prassad S.P. College of Veterinary Science, Birsa Agricultural University, Ranchi, Bihar-834 007, India. 1Present address: Institute of Animal Health-cum-Biological Products, Zakura, Srinagar-190 006, India. Abstract Oocytes Liberated from abattoir goat ovaries were cultured in tissue culture medium-199, supplemented with heat inactivated oestrus cow serum, at 39°C for 24 hours. A total of 225 cultured oocytes were studied for nuclear maturation with 40.44% oocytes maturing to metaphase-II, 28.81% in different stages of maturation (mataphase-I to telophase-I), 14.66 in germinal vesicle stage and 16% oocytes were observed abnormal. Top | |
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