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Indian Journal of Agricultural Biochemistry
Year : 2014, Volume : 27, Issue : 2
First page : ( 165) Last page : ( 169)
Print ISSN : 0970-6399. Online ISSN : 0974-4479.

Analysis of Genetic Diversity among Brinjal (Solanum melongena L.) Genotypes through Dominant Markers

Thakkar J R*, Mandavia M K, Golakiya B A, Sabara P H

Department of Biotechnology, Junagadh Agricultural University, Junagadh-362001, Gujarat, India

*Author for correspondence: Email: thakkarjalpa@yahoo.com

Online published on 16 January, 2015.

Abstract

Ten Brinjal genotypes were analyzed for their genetic diversity study through nineteen RAPD and ten ISSR primers among which ten RAPD and six ISSR primers gave good polymorphism with 83.43 and 81.03 percent average polymorphism, respectively. The RAPD primers produce 480 amplified products with 71 bands among which 57 were polymorphic, giving average PIC and resolving power value of 0.837 and 3.48, respectively. The ISSR primers produced 52.17 average numbers of amplified products with 6.33 and 7.83 average number of polymorphic and total numbers of bands. The ISSR primers gave average PIC and resolving power value of 8.44 and 2.83, respectively. The RAPD primers produced band size range from 150–2659 while ISSR produced 113–3744. Jaccard's similarity range for RAPD, ISSR and pooled data were 0.50 to 0.77, 0.55 to 0.86 and 0.52–0.75. Different combinations of similarity matrix and cophectic values of RAPD, ISSR and pooled data showed good correction through mantel's test (r=0.799 to 0.881) except for ISSR (r=0.486 to 0.463). Results indicated RAPD as a better marker and helped to study relatedness of different genotypes for future developments.

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Keywords

Genetic diversity, brinjal, RAPD, ISSR.

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