Factors affecting Callus Induction in Mothbean [Vigna aconitifolia (Jacq.) Hande V. P.1, Chauhan R. M.1, Dapke J. S.2,*, Vanave P. B.2 1Department of Genetics & Plant Breeding, C. P. College of Agriculture SDAU, Sk Nagar, India 2Department of Genetics & Plant Breeding, N. M. College of Agriculture, NAU Navsari, India *For Correspondence-jitudapke@gmail.com
Online published on 16 December, 2015. Abstract Mothbean [Vigna aconitifolia (Jacq.) Marechal] belongs to fabaceae family is an important food grain legume crop that is cultivated over the entire world. Five varieties of mothbean viz., GMO 1, GMO 2, CZM 2, Jwala and RMO 40 were investigated for callus induction. Cent per cent callus induction was obtained in both the explants of five varieties on different medium combinations tested. Significant differences were observed among the varieties, explants, medium combinations and their two as well as three way interactions for both the days taken for callus initiation and callus fresh weight except for variety × explant × medium interaction in case of days taken for callus initiation. Among the different medium combinations tried, MS + 2.0 mg l−1 2, 4-D + 1.0 mg l−1 BAP recorded significantly minimum number of days (6.356) for callus initiation and produced the highest callus fresh weight (2.457 g). Of the different varieties tested, Jwala took minimum number of days to callus initiation (6.944) and produced the highest callus fresh weight (2.056 g). Among the explant types, the hypocotyl explants recorded the least number of days to callus initiation (7.660) and leaf explants produced the highest callus fresh weight (1.932 g). Considering the interaction effects, MS + 2.0 mg l−1 2, 4-D + 1.0 mg l−1 BAP for days to callus initiation and callus fresh weight were identified as the most suitable media for callus induction. MS medium supplemented with 1.0 mg l−1 2, 4-D promoted the growth of friable and organized callus in all the varieties and hence, this medium was identified as the most suitable medium for callus maintenance. Top Keywords In vitro, mothbean, callus culture. Top |