Use of Soybean Oil Fry Waste for Economical Biosurfactant Production by Isolated Pseudomonas aeruginosa Using Response Surface Methodology de Lima C. J. B., Contiero J.* Department of Biochemistry and Microbiology, Biological Sciences Institute, Rio Claro, São Paulo, University - Unesp - CEP 13506-900 Rio Claro, São Paulo, Brazil *For correspondence - jconti@rc.unesp.br
Abstract The present study sought biotensoactive production from soybean oil fry waste using Pseudomonas aeruginosa ATCC 10145 and Pseudomonas aeruginosa isolated from the soil of a petroleum station having undergone gasoline and diesel oil spills. The results of the experiments were analyzed using a complete factorial experimental design, investigating the concentration of soybean oil waste, ammonia sulfate and residual brewery yeast. Assays were performed in 250-mL Erlenmeyer beakers containing 50 mL of production medium, maintained on a rotary shaker at 200 rpm and a temperature of 30±1°C for a 48-hour fermentation period. Biosurfactant production was monitored through the determination of rhamnose, surface tension and emulsification activity. The Pseudomonas aeruginosa ATCC 10145 strain and isolated Pseudomonas aeruginosa were able to reduce the surface tension of the initial medium from 61 mN/m to 32.5 mN/m and 30.0 mN/m as well as produce rhamnose at concentrations of 1.96 and 2.89 g/L with emulsification indices of 96% and 100%, respectively. Top Keywords Pseudomonas aeruginosa, Biosurfactant, Rhamnose, Surface-active, Emulsification index, Soybean oil. Top |