Differential pulse polarographic studies of levofloxacin antibiotic and its determination in pharmaceutical formulations and biological samples Masram Chetankumar B., Jugade R. M.1 S.N. Mor College, Tumsar, Maharashtra441912 1Department of Chemistry, RTM Nagpur University, Nagpur-440033 Online published on 6 October, 2021. Abstract Levofloxacin, a well-known laevorotatory isomer of antibiotic ofloxacin was studied for electrochemical activity on dropping mercury electrode. It gave well-defined symmetrical peak in Britton-Robinson buffer at pH 5.0 with peak potential, Ep =-1.314 V. Effect of various parameters including pH, scan rate, pulse amplitude, scan range etc were optimized. Under optimum conditions of pH 5.0, scan rate of 12 mV/s, pulse amplitude of 100 mV and scan range of-0.6 V to-1.6 V, the linear correlation between peak current and concentration extended from 0 to 1000 ppm with 3o detection limit OF 0.036 ppm. The method thus developed was applied for the estimation of levofloxacin in pharmaceutical formulations as well as urine and blood samples. Top Key Words Levofloxacin, Differential pulse polarography, Dropping mercury electrode. Top |