Calf fecal origin Limosilactobacillus reuteri and Helianthus tuberosus synbiotic improved gut health with calf fecal inoculum using In vitro hind gut model Lakshmi K.S.R., Kala Anju*, Verma A.K., Chaudhary L.C., Agarwal Neeta, Ali Md. Younus1, Shindhe Bharath Animal Nutrition Division, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, India 1SAARC Agriculture Centre, BARC Complex, Farmgate, Dhaka-1215, Bangladesh *Corresponding author: anjukalavet2002@gmail.com
Online published on 2 January, 2025. Abstract This study aimed to evaluate a synbiotic product as a growth promoter for young calves using autochthonic lactic acid bacteria (LAB) isolates as probiotics in conjunction with Jerusalem artichoke (Helianthus tuberosus) tuber (JAT) powder under in vitro conditions. Ten LABs isolates underwent rigorous screening for their probiotic attributes like low pH and bile salt tolerance, cell surface hydrophobicity, autoagglutination ability, antibiotic sensitivity, biofilm formation, and anti-pathogen activity. Based on the preliminary screening results, the best three isolates (RM102, RM119 and RM125) selected and identified as Lactiplantibacillus plantarum Pediococcus pentosaceus, and Limosilactobacillus reuteri, via 16S rRNA sequence analysis. In the subsequent phase, JAT powder was incorporated at varying concentrations (0, 2, 4, 6, 8, and 10 mg/mL) in the anaerobic fecal inoculum using in vitro hindgut model (IHM). Post 24 h incubation, the fermented fecal inoculum was assessed for changes in log CFU of Lactobacilli, Bifidobacteria, Coliforms, Salmonella and biochemicals. The biochemical responses displayed a linear improvement (P<0.001) with increasing prebiotic dosage. The three chosen probiotic isolates paired with JAT powder were individually subjected to 24h incubation using IHM to assess the potential of synbiotic. The results revealed a synbiotic combination of Limosilactobacillus reuteri RM125 (1.5-3.0 x 108 cfu/ mL) with 10 mg/mL JAT powder as the best combination for neonatal calf feeding. Top Keywords Hindgut microbiota, In vitro hindgut model, Jerusalem artichoke, Lactic acid bacteria Neonatal calves, Synbiotic. Top |