Isolation and Characterization of Bacterial Leaf Blight of Rice (Xanthomonas oryzae pv. oryzae) Isolates from Southern India Shankara K.1,*, Patil M. B.1, Pramesh D.2, Sunkad Gururaj1, Chikkannaswamy1 1Department of Plant Pathology, University of Agriculture Sciences, Raichur, Karnataka 2Rice Pathology, ARS, Gangavathi, Karnataka *Email: pathshankar.k@gmail.com
Online published on 2 January, 2017. Abstract Bacterial leaf blight (BLB) of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major biotic constraint in rice cultivation and is wide spread in Asia, including India. A total of 54 isolates were collected from different rice growing zones of southern India. All the isolates were obtained and subjected to pathogenicity test on BPT-5204 that varied significantly in terms of disease severity among each other and further confirmed withcolony morphological features and biochemical tests. Colony size of the isolates ranged from 1.2 mm to 4.0 mm with light yellow to yellow and creamy yellow colour with circular to irregular margin. Most of the isolates produced raised, slimy colonies but few isolates produced flattened and slimy colonies. All the isolates were subjected to different biochemical tests and were found to be positive for 3 per cent KOH test, gelatin liquefaction, catalase test, while, negative for starch hydrolysis and oxidase tests. Five isolates namely, Xoo7, Xoo16, Xoo22, Xoo27 and Xoo50 found negative for H2S production test. Top Keywords Bacterial leaf blight, Biochemical characterization, BLB, Rice, Morphology, Xanthomonas oryzae pv. oryzae. Top |