Isolation, Optimization and Purification of Acidic Nitrilase from Strain AP 5 Pratush Amit1,*, Gupta Anupama2, Kumar Ajay3, Kumar Amit3, Patil Sandip4, Singh Rajinder1, Kumari Preeti1, Priya1 1Department of Biotechnology, Shoolini Institute of Life Sciences and Business Management, The Mall, Solan. 2Department of Microbiology, UHF, Nauni 3Department of Microbiology, Shoolini Institute of Life Sciences and Business Management, The Mall, Solan 4Department of Microbiology, Shoolini University, Solan *Email: amit.pratush@gmail.com
Online published on 16 February, 2013. Abstract Strain AP 5 is a Gram +ve, cocci and non spore forming in nature and has been reported to produce nitrilase enzyme that converts benzonitrile to benzamide. The production as well as the reaction conditions and purification of nitrilase enzymes from this strain has been optimized in present article. The strain AP 5 produce nitrilase enzyme (3.2 U/mg dcw) in M5 medium at pH 6.0. Under the optimized reaction conditions the strain AP 5 exhibited maximum nitrilase activity [6.5 U/mg dcw (milligram dry cell weight)] at 30 °C in 0.1M potassium phosphate buffer (pH 5.5). the enzyme was purified up to 1.5 fold of purification with an yield of 40%. Top Keywords Gram +ve, nitrilase activity, optimization, strain AP 5. Top |