Screening for Anticancer Activity of Novel Polyenzyme Formulations using In Vitro Methods Joshi Anita1, Risbud Shilpa1, Juvekar Aarti2,,* 1Advanced Enzyme Technologies Ltd. Thane 2Advanced Centre For Treatment, Research and Education in Cancer (ACTREC), Cancer Research Institute, Kharghar, Navi Mumbai, 410 208 *Email: ajuvekar@actrec.res.in
Abstract The screening for anticancer activity of five formulations developed by Advanced Enzyme Technologies Ltd., Thane was done at the ACTREC, Navi Mumbai. These are novel polyenzyme formulations with defined mixtures of enzymes of microbial, fungal, plant and animal origin. These candidate formulations were tested for anticancer activity by the Sulphorhodamine B assay and a panel of ten human malignant cell lines was used which was derived from different tissues such as ovary, colon, prostate, oral, lung, breast, cervix and leukaemias. It was found that the formulation ABL-1 showed broad-spectrum activity since it produced activity against nine of the ten cell lines. It appears to show better potency against breast, ovarian and oral cell lines. The difference in reactivity of these formulations makes it clear that they have a specific mode of action and have not inhibited cells non-specifically. Preliminary in vivo studies using the BDF mice showed that the formulations were non-toxic Further in vivo studies need to be undertaken for efficacy determination. The formulations therefore show good potential for complementary cancer therapy. Top Keywords Polyenzyme formulation, novel anticancer agents, lead identification, broad spectrum, in vitro studies using malignant cell lines. Top |