Development of New Spectrophotometric Methods for Quantitative Determination of 7-ADCA in Pharmaceutical Formulations Kishore Medikondu*, Kalyani Ch. S.R.G. Department of Post-Graduate Chemistry, SVRM College and Research Center, Nagaram, Guntur (District), Andhra Pradesh, India-522268 *Corresponding Author E-mail: medikissi@gmail.com
Online published on 6 March, 2013. Abstract Three simple, sensitive and accurate methods are described for the determination of 7-Amino deacetoxy cephalosporanic acid (7-ADCA) in bulk drug and in formulations. Methods Ma to Mc are based on redox/charge transfer reaction (Maandb) and Redox reaction (Mc) between 7-ADCA and N-Bromosuccinamide/p-N-methyl aminophenol and p-Sulphanilic acid (NBS/PMAP/SAc) (Ma), Haematoxylin/Chloramine T (Hae/CAT)(Mb) and NBS/Celestine blue (NBS/CB) (Mc) solutions. The chromogen being extractable with chloroform could be measured quantitatively at 560 (Ma) 630 (Mb) and 520 nm (Mc). All variables were studied to optimize the reaction conditions. Regression analysis of Beer's Law plot showed good correlation in the concentration ranges 4–24 for Ma, 8–48 for Mb and 8–48 μg/ml for Mc. The calculated molar absorptivityvalues are 7.205 x 103, 5.404 x 103, and 2.899 x 103l/mol/cm for Ma to Mc, respectively. The methods were successfully applied to the determination of 7-ADCA in formulations and the results tallied well with the label claim. The results were statistically compared with those of a literature method by applying the student's t-test and F-test. No interference was observed from the concomitant substances normally added to preparations. The accuracy and validity of the methods were further ascertained by performing recovery experiments via standard-addition method. Top Keywords 7-Amino deacetoxy cephalosporanic acid, Redox, Charge transfer reactions, spectrophotometric methods, statistical analysis, recovery studies. Top |