Asian Journal of Research in Chemistry
Year : 2009, Volume : 2, Issue : 4
First page : ( 485) Last page : ( 487)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.

RP-HPLC Determination of Atenolol in Its Pharmaceutical Dosage Forms

Sharma Amrish*, Tailang Mukul, Khare Sonali, Shukla Tripti

People's Institute of Pharmacy and Research Centre, Bhanpur, Bhopal-462 037 (M.P.)

*Corresponding Author E-mail: amrish_sharma21@rediffmail.com

Online published on 13 March, 2013.

Abstract

A new and simple reverse phase HPLC method was developed for the estimation of atenolol in its pharmaceutical dosage forms. The mobile phase used acetonitrile, methanol, KH₂PO₄ is in the ratio of 250:250:500. Buffer solution was prepared by dissolving 0.05M KH₂PO₄ + 0.1% H₃PO₄ adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini(250× 4.6mm) and 5μ particle size with rheodyne injector. The flow rate was 1ml/min and uv detection at 238nm. The retention time for atenolol was 4min respectively. The linearity coefficient of atenolol was found to be 0.99% and the percentage recoveries for atenolol is 99.89%. The proposed method was accurate, precise and linear within the desired range. This method can successfully employed for the quantitative analysis of atenolol.

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Keywords

RP HPLC, Validation, Atenolol.

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