Biodecolourization of Direct Orange (WS) 102 dye by isolated white and green fungus and characterization of ligninperoxidase from fungus Damahe Swati1, Choube Pallavi1, Katolkar Parimal2,* 1Department of Biotechnology, Kamla Nehru College, Sakkardara Square, Nagpur-440009 2Manoharbhai Patel Institute of Pharmacy (B. Pharm.), Kudwa, Gondia-441614 *Corresponding Author E-mail: p_k_7995@yahoo.co.in
Online published on 5 June, 2014. Abstract The aim of present study was to screen of fungus capable of degrading and decolorizing Direct Orange 102 dye. The said fungus was isolated from soil of dumping area near Nagpur (MH) India. Decolourization of fungus carried out by isolation of fungus by serial dilution of soil followed by pour plate technique was followed the colonies showing zone of decolourization are picked for further study. The isolated fungus was identified by macroscopic and microscopic study. Isolated fungus was named as green fungi and white fungi. This fungus showed decolourization at room temperature under static condition. Enzyme assay was performed at different weather conditions. The decolourization was due to bioabsorption or biodegradation, fungus were grown in liquid culture growth medium using bagasse particles as a natural lignin source using n-propanol as a substrate at wavelength 300 nm, spectrophotometrically. The enzymatic characteristics (pH and temperature) of ligninperoxidase were studied using n-propanol as substrate. The isolated fungus was co-immobilized in Ca-alginate beads and their decolourization efficiency was determined. The isolated bacteria and fungus were immobilized and showed decolourizing activity. Top Keywords Biodecolourization, Direct Orange (WS) 102 dye, Enzyme assay, Lignineperoxidase, Green and White fungi. Top |